Routine proteomic analyses

We provide a routine service in the field of proteomics for the need of our colleagues at IOCB. Our group is involved in numerous projects where the MS-based proteomic approaches is required. These projects deal with, for example, the research of Hepatitis B viral infection or analysis of rhomboid dependent proteome changes, etc.

We provide following proteomic analyses:
  • Intact mass analysis

            molecular weight determination of protein or oligonucleotides by MALDI-TOF or ESI-TOF

  • Protein identification

            including protein complexes characterization from gel or solution

  • Characterization of posttranslational modifications

            phosphorylation, acetylation, methylation etc.

  • Protein quantification
            relative quantification (label-free, SILAC labeling)

Proteomic research

In our research projects we focus on:

  • analysis of lipoproteins/lipopeptides and membrane proteins

Separation and identification of lipoproteins/lipopeptides is rather challenging due to the limited solubility of lipo-modified segments in water-based solutions, whose are exclusively used within standard proteomic workflows. Therefore, we focus on the implementation of new workflows to the increase the yield of lipo-modified and hydrophobic segments of the proteins.

  •  implementation of novel structural proteomics approaches. In the field of structural proteomics, we implement the following methods:

native MS

protein covalent labelling

protein crosslinking

hydrogen-deuterium exchange MS

These complementary methods allow to study protein structures and characterize not only protein-protein interactions but also interactions of proteins with other biological molecules.