Routine proteomic analyses
We provide a routine service in the field of proteomics for the need of our colleagues at IOCB. Our group is involved in numerous projects where the MS-based proteomic approaches is required. These projects deal with, for example, the research of Hepatitis B viral infection or analysis of rhomboid dependent proteome changes, etc.
- Intact mass analysis
molecular weight determination of protein or oligonucleotides by MALDI-TOF or ESI-TOF
- Protein identification
including protein complexes characterization from gel or solution
- Characterization of posttranslational modifications
phosphorylation, acetylation, methylation etc.
- Protein quantification
In our research projects we focus on:
- analysis of lipoproteins/lipopeptides and membrane proteins
Separation and identification of lipoproteins/lipopeptides is rather challenging due to the limited solubility of lipo-modified segments in water-based solutions, whose are exclusively used within standard proteomic workflows. Therefore, we focus on the implementation of new workflows to the increase the yield of lipo-modified and hydrophobic segments of the proteins.
- implementation of novel structural proteomics approaches. In the field of structural proteomics, we implement the following methods:
protein covalent labelling
hydrogen-deuterium exchange MS